Demonstration of mRNA using digoxigenin labelled oligonucleotide probes for in situ hybridisation in formamide free conditions.
نویسندگان
چکیده
منابع مشابه
Demonstration of mRNA using digoxigenin labelled oligonucleotide probes for in situ hybridisation in formamide free conditions.
The value of formamide for use in in situ hybridisation (ISH) for the detection of mRNA, using either single or multi probe cocktails of digoxigenin labelled oligoprobes, was investigated. Three peptides with cell specific localisation in three separate tissues--calcitonin in the thyroid, epidermal growth factor (EGF) in the submaxillary gland, and insulin in the pancreas were studied. In each ...
متن کاملSensitivity of digoxigenin and biotin labelled probes for detection of human papillomavirus by in situ hybridisation.
The sensitivity of digoxigenin and biotin labelled DNA probes for the detection of human papillomavirus (HPV) by dot blotting and in situ hybridisation was compared in tissues from cervical, laryngeal, and anogenital neoplasia. Probes were either labelled with digoxigenin by the random primer technique and detected with anti-digoxigenin antibody, or labelled with biotin by nick translation and ...
متن کاملDetection of mRNA by in situ hybridisation and in northern blot analysis using oligodeoxynucleotide probes labelled with alkaline phosphatase.
AIMS To assess whether a reduction in intensity of signal observed using an alkaline phosphatase labelled oligodeoxynucleotide probe could be explained on the basis of procedural steps rather than reduced sensitivity. METHOD Signal intensity was assessed on in situ hybridisation for pro-opiomelanocortin (POMC) mRNA in rat pituitary and for somatostatin mRNA in human pancreas and in northern b...
متن کاملSynthesis and evaluation of phosphorescent oligonucleotide probes for hybridisation assays.
Monofunctional, p-isothiocyanatophenyl-derivatives of platinum (II)-coproporphyrin-I (PtCP-NCS) were evaluated as phosphorescent labelling reagents for synthetic oligonucleotides containing a 3'- or 5'-amino modification. Synthesis and purification conditions were optimised to generate high yields and purity of PtCP-labelled oligonucleotide probes. Phosphorescent properties of the PtCP label ha...
متن کاملIn situ hybridization with oligonucleotide probes.
Equipment and reagents • Glass coverslips, 22x22mm • 6-well plates • 4% freshly made paraformaldehyde (Electron Microscopy Sciences) in PBS, pH 7.4 • Triton X-100 (Sigma) • Parafilm • Forceps, Dumont, GG (Electron Microscopy Sciences) • Avidin-DCS-Texas Red or –fluorescein (Vector) • Filter paper • Mounting medium (Molecular Probes) • Microscopy coverslide • Deionized formamide (Ambion) • Yeast...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1993
ISSN: 0021-9746
DOI: 10.1136/jcp.46.2.171